PLEX-ID Broad Viral I Assay
The PLEX-ID Broad Viral I assay is a qualitative, non-diagnostic assay intended for the detection and identification of common viruses from several important groups, from direct specimens through nucleic acid amplification and subsequent mass spectrometry analysis. The PLEX-ID Broad Viral I assay is designed to detect and identify individual organisms or mixtures of Herpesviridae, Human adenovirus, Human enterovirus, Polyomavirus and Parvovirus direct from sample.
| Name | List No. | Quantity | Additional Information |
| PLEX-ID Broad Viral I (PLEX-ID) | 06N34-61 | 10 plates (120 assays) |  |
| Magnetic Bead particles | 04N77-02 | 4 bottles | |
| Clean-up Reagents | 04N77-01 | Reagents 1,2,3 | |
| PLEX-ID Broad Viral I* (T5000) | 06N34-01 | 10 plates (120 assays) |
*Magnetic beads and clean-up reagents are included with the T5000 assay but must be purchased separately for use with the PLEX-ID assay.
The PLEX-ID Broad Viral I assay kit includes:
- 10 Plates (120 Assays)
- Plate Processing Protocol
- RT-PCR Dilution Buffer
- RNA Dilution Buffer
- Superscript® III Reverse Transcriptase
- T-4 Gene 32 Protein
A clean-up kit is included with the T5000 assay but must be purchased separately for use with the PLEX-ID assay.
Positive, negative, or extraction controls are not provided.
The PLEX-ID Broad Viral I assay uses fourteen primer pairs multiplexed into eight wells to detect and identify the following viruses:
- Herpesviridae
- HSV-1 (HHV-1) and HSV-2 (HHV-2)
- CMV (HHV-5)
- VZV (HHV-3)
- EBV (HHV-4)
- HHV-8
- Human adenovirus species A-F
- Human enterovirus species A-D
- Polyomavirus
- BK virus
- JC virus
- Parvovirus B19
The assay is configured to identify 250 species and subspecies of the targeted viral groups as well as variants and related emerging organisms.
Identification is reported based upon detection of the specific viruses listed. Detection outside of the targeted organism list, such as variants with novel basecount signatures, are reported according to the closest known species in the database.
