The PLEX-ID Broad Fungal assay is a qualitative, non-diagnostic assay intended for the detection and identification of important fungal species from isolates, through nucleic acid amplification and subsequent mass spectrometry analysis.
*Magnetic beads and clean-up reagents are included with the T5000 assay but must be purchased separately for use with the PLEX-ID assay.
The PLEX-ID Broad Fungal assay kit is comprised of:
- 10 Plates (60 Assays)
- Plate Processing Protocol
- DNA Dilution Buffer, 8ml (2x)
Positive, negative, or extraction controls are not provided.
The PLEX-ID Broad Fungal assay uses twelve primer pairs multiplexed into sixteen wells to detect and identify the following threat agents:
| Organism | Organism |
| Ajellomyces capsulatus (Histoplasma capsulatum) | Candida krusei |
| Aspergillus spp. | Candida parapsilosis |
| Aspergillus flavus | Candida tropicalis |
| Aspergillus fumigatus | Clandophialophora bantiana |
| Aspergillus | Clavispora lusitaniae |
| Aspergillus terreus | Coccidiodes immitis |
| Bipolaris hawaiiensis | Cryptococcus spp. |
| Bipolaris spicifera | Cryptococcus neoformans var. neoformans |
| Blastomyces dermatitidis | Cryptococcus neoformans var. gattii |
| Candida spp. | Curvularia pallescens |
| Candida albicans | Exophiala dermatitidis |
| Candida dubliniensis | Pneumocystis carinii |
| Candida glabrata | Pneumocystis jiroveci |
| Fusarium oxysporum | Ramichloridium mackenzei |
| Fusarium solani | Rhizopus spp. |
| Penicillium marneffei |
This assay is designed to identify and report the organisms listed above. Other organisms identified outside of those above are reported at the species or genus level.
