The PLEX-ID Broad Bacteria Lo assay is a non-diagnostic assay designed to detect and identify all groups of bacteria, including intracellular organisms like Mycoplasma, Chlamydia, and Rickettsia as single organisms or simple mixtures from isolates or high-load samples, through nucleic acid amplification and subsequent mass spectrometry analysis. This assay offers the same coverage as the PLEX-ID Broad Bacteria Assay but is suitable for analysis of direct specimens from sterile fluids or other matrices.
| Name | List No. | Quantity | Additional Information |
| PLEX-ID Broad Bacteria Lo (PLEX-ID) | 03N34-63 | 10 plates (60 assays) |  |
| Magnetic Bead particles | 04N77-02 | 4 bottles | |
| Clean-up Reagents | 04N77-01 | Reagents 1,2,3 | |
| PLEX-ID Broad Bacteria Lo* (T5000) | 03N34-03 | 10 plates (60 assays) |
*Magnetic beads and clean-up reagents are included with the T5000 assay but must be purchased separately for use with the PLEX-ID assay.
The PLEX-ID Broad Bacteria assay kit includes:
- 10 Plates (60 Assays)
- Plate Processing Protocol
- PCR Enzyme Dilution Buffer
- DNA Dilution Buffer
- Abbott Taq Gold Polymerase
A clean-up kit is included with the T5000 assay but must be purchased separately for use with the PLEX-ID assay.
Positive, negative, or extraction controls are not provided.
The PLEX-ID Broad Bacteria assay uses 16 primer pairs multiplexed into sixteen wells to detect and identify the following:
- More than 3,100 species of bacteria
- Gram-positive Firmicutes
- Staphylococcus speciation
- Gram-positive Bacilli
- Bacillus speciation
- Gram-negative Enterobacteria
- Gram-negative Gamma Proteobacteria
- Gram-negative Betaproteobacteria
- Gram-negative Beta and Gamma Proteobacteria
This assay is designed to be able to identify more than 3,100 species of bacteria with identity reported as a single species or as a cluster of known species. Unknown organisms detected with novel basecount signatures are reported according to the closest known species in the database.
Additional Information
- Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates. CD Baldwin, et al. Diagnostic Microbiology and Infectious Disease. 2009. 64: 403-408.
- Identification of Bacterial Plant Pathogens Using Multilocus Polymerase Chain Reaction/Electrospray Ionization-Mass Spectrometry. E Postnikova et al. Phytopathology. 2008. 98(11): 1156-1164.
- Rapid Identification and Strain-Typing of Respiratory Pathogens for Epidemic Surveillance. DJ Ecker et al. Proceedings of the National Academy of Sciences (USA). 2005. 102(22): 8012-8017.
